Comparison of three kinds of blood and two incubation atmospheres for cultivation of Bordetella pertussis on charcoal agar.

Comparison of four charcoal media for the isolation of Bordetella pertussis.

Charcoal-horse blood agar with 40 micrograms of cephalexin per ml, charcoal-horse blood agar with 3 micrograms of lincomycin per ml, charcoal agar with 3 micrograms of lincomycin per ml, and Legionella (buffered charcoal-yeast extract) agar with 3 micrograms of lincomycin per ml were compared for isolation of Bordetella pertussis. Charcoal-horse blood agar with 40 micrograms of cephalexin per m...

Use of supplemented Stainer-Scholte broth for the isolation of Bordetella pertussis from clinical material.

The use of Stainer-Scholte broth supplemented with (2,6-O-dimethyl)beta-cyclodextrin (heptakis) for the isolation of Bordetella pertussis from clinical specimens was evaluated with 3,632 nasal swabs from children and adults with suspected whooping cough or from their family contacts. The liquid enrichment medium was subcultured on charcoal agar with 10% defibrinated horse blood. Charcoal agar a...

Susceptibility testing of Bordetella pertussis.

Korgenski and Daly recommend charcoal horse blood agar (Regan-Lowe agar) for the detection of erythromycin resistance in Bordetella pertussis by agar dilution, Etest methodology, or agar diffusion. MICs for most isolates were #0.06 to 0.12 mg/ml by agar dilution and the Etest, while the MIC for the only resistant strain was 16 to 64 mg/ml (4). Charcoal agar may be suitable for discrimination be...

Isolation of Bordetella pertussis: benefits of using both Bordet-Gengou and charcoal agar media.

جداسازی بوردتلاپرتوسیس و پاراپرتوسیس از نمونه‌های بالینی جمع‌آوری شده از سراسر ایران

Background and purpose: Bordetella pertussis is a gram negative and obligate aerobic bacteria that is cause of whooping cough and is exclusively a human pathogen. In the last decade increasing rate of pertussis was observed. Despite the importance of pertussis as a contagious disease enough information does not exist regarding its incidence rate in Iran. In this research pertussis suspicious ...